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After 20 minutes, the absorbance of the mixture was read at 517nm.

  • According to the results, compounds 2a, 3a, 3c and 9 showed antibacterial activity against all tested bacteria and compound 3c had the highest activity with inhibition zones ranged 13-16 mm Table 1.

  • The discs were placed aseptically on the surface of the inoculated solidified plates at equal distances.




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